LOCUS       pLV-[hsa-miR-1279]        9116 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7037..7897
                     /label=Amp(R)
     promoter        8098..8638
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5642..5876
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6451..6906)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5948..6078
                     /label=SV40 pA
     misc_feature    6079..6292
                     /label=SV40 ori
     misc_feature    4963..5559
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3581
                     /label=intron
     CDS             3628..4953
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3371
                     /label=hsa-mir-1279 precursor
BASE COUNT     2198 a      2356 c      2437 g      2125 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg ctgcatactc 
     3121 agtgaatgcc acaacctgaa attctaaaaa aagaacattg gttaagacaa acctaaatca 
     3181 atctgaacag tgttgcctga atatttagaa tattcacaaa aattcatatt gcttctttct 
     3241 aatgccaaga aagaagagta taagaacttc ctcccagaag cctagacatt ttaatacatt 
     3301 ttctatttca acttacaatg aaactttaat cctgaatctt gatttatcta aatcaaaaat 
     3361 ctcacattac ggctagctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt 
     3421 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc 
     3481 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca 
     3541 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga aaactacccc 
     3601 tctagagtcg agctaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc 
     3661 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 
     3721 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 
     3781 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 
     3841 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc 
     3901 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 
     3961 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 
     4021 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 
     4081 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag 
     4141 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc 
     4201 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag 
     4261 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 
     4321 gacgagctgt acaaggaccc accggtcgcc accatgaccg agtacaagcc cacggtgcgc 
     4381 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 
     4441 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 
     4501 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 
     4561 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 
     4621 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 
     4681 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 
     4741 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 
     4801 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 
     4861 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg agtgcccgaa ggaccgcgcg 
     4921 acctggtgca tgacccgcaa gcccggtgcc tgagcggccg cgtcgacaat caacctctgg 
     4981 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 
     5041 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 
     5101 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 
     5161 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 
     5221 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 
     5281 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 
     5341 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 
     5401 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 
     5461 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 
     5521 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt 
     5581 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 
     5641 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc 
     5701 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
     5761 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
     5821 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 
     5881 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 
     5941 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     6001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     6061 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta 
     6121 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 
     6181 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 
     6241 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg 
     6301 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 
     6361 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 
     6421 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg 
     6481 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 
     6541 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 
     6601 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 
     6661 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 
     6721 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 
     6781 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 
     6841 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta 
     6901 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     6961 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     7021 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     7081 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     7141 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     7201 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     7261 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     7321 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     7381 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     7441 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     7501 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     7561 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     7621 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     7681 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     7741 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     7801 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     7861 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     7921 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     7981 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     8041 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     8101 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     8161 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 
     8221 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     8281 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     8341 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     8401 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     8461 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     8521 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     8581 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     8641 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     8701 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 
     8761 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 
     8821 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 
     8881 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 
     8941 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 
     9001 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 
     9061 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgc 
//
